- Functional groups
- Non-covalent interactions
- Digestion of biomolecules
- Glycolysis and fates of pyruvate
- Krebs cycle
- Cellular respiration
- Glycogen metabolism and gluconeogenesis
- Pentose phosphate pathway
- Fatty acids metabolism
- Cholesterol metabolism
- Aminoacids metabolism
Friday, November 30, 2012
Tuesday, November 27, 2012
Saturday, November 24, 2012
Monday, November 19, 2012
The geometric isomers belong to the family of the stereoisomers, more precisely, the configurational isomers. So, as the name indicates, they differ in the configuration of one or more carbons. The particularity of this kind of isomerism is the fact that it involves non-tetraedric carbons, that means, carbons that do not establish 4 single bonds. Putting it more simply, it involves carbons that establish double bounds.
The classic example of this kind of isomerism is the cis and trans isomers, where the configuration of one of the carbons involved in the double bound changes. It is important to note that these isomers are not conformational ones, because the double bonds cannot rotate.
Finally, it is noteworthy that one should be careful when comparing two molecules that differ in a region that contains double bonds. In this case, the molecules are geometric isomers only if the constitutional isomerism is not applicable to the molecules. For example, in the following image the first two molecules are geometric isomers, while the molecules 1 and 3, or 2 and 3 are constitutional isomers.
Thursday, November 15, 2012
PCR is present in the daily routine of many investigators. Due to Dr. Ahern (www.davincipress.com/
metabmelodies.html), it can also be present in the form of a music. This sob was base on the famous She’ll Be Comin’ Around the Mountain.
First you must design the primers- PCR!
Make sure that they won't form dimers - PCR!
Then you check the melting T's
A's and T's are two degrees
And it's four for G's and C's in PCR!
Oh the thermocycler's set for PCR!
If this fails you'll be upset with PCR!
First you melt and did I mention
It's anneal and then extension
Copying is the intention - PCR!
Many times the protocol you've tried to fix
Checking all your tubes and then your mastermix
Looking for contamination
As you build up your frustration
If there's no amplification - PCR!
You can use reverse transcription - PCR!
Surely you'll throw a conniption - PCR!
Other types may give you trouble
Oh there's inverse and there's bubble
Your anxiety will double - PCR
Oh you've really got to amplify this strand
Hope that when you run your gel you've got a band
Didn't get what you expected
Damn this project you selected
Now you're feeling quite dejected - PCR!